Evaluation of Cis-Regulatory Elements of Dicot ?-TbSt Promoter

Abstract

The eukaryotic gene expression is controlled by a regulatory region called promoter. Many plant promoters have been characterized for regulatory motifs. There are three types of plant promoters i.e. inducible, constitutive and tissue specific on basis of regulatory motifs. Plant sources have been searched for isolation of strong promoters that are being utilized in molecular biology research. The researchers need to address IPR issues for utilizing the strong patented promoters for the expression of their transgenes. The identification and characterization of strong dicot promoter is necessary for the expression of transgenes by native researchers to evaluate their artificial gene. The promoters isolated from viral sources have some limitations. The dicot promoter sequence of ?-tubulin (?-TbSt) was explored and isolated from potato. The ?-TbSt promoter sequence consists of light responsive, hormonal responsive and stress responsive elements. Motifs having responsive elements were identified in ?-TbSt promoter. The ?-TbSt promoter is highly constitutive promoter.