Exploring Beta-Lactamase Inhibitory Potential of Common Medicinal Plants

Authors

  • Areej Fatima Institute of Microbiology and Molecular Genetics, University of the Punjab, Quaid-e-Azam Campus, Lahore-54590, Pakistan.
  • Amina Javid Institute of Microbiology and Molecular Genetics, University of the Punjab, Quaid-e-Azam Campus, Lahore-54590, Pakistan.
  • Mehboob Ahmed Institute of Microbiology and Molecular Genetics, University of the Punjab, Quaid-e-Azam Campus, Lahore-54590, Pakistan. https://orcid.org/0000-0002-6363-9050

DOI:

https://doi.org/10.52700/jmmg.v5i1.153

Keywords:

Antibiotic resistance, ?-Lactamases inhibitors, Pharmacoinformatics, Molecular docking, Time-kill assay

Abstract

The use of ?-Lactam antibiotics improperly has significant negative effects on public safety and health around the world. It causes the disturbance of gut flora, the emergence of multi-drug-resistant bacteria, and environmental contamination. The focus of this investigation was the Gram-negative pathogens Escherichia coli (E. coli), and Pseudomonas aeruginosa (P. aeruginosa). Potent beta-lactamase inhibitors were found among phytochemicals from medicinal plants by in-silico analysis, with Gallocatechin gallate from Green tea and Glycyrrhetinic acid from Licorice being particularly effective against TEM-1 and IMP-1 ?-Lactamases, respectively. In-vitro analysis such as D-zone assay and time-kill assay showed that the methanolic plant extracts improved the effectiveness of antibiotics against these Gram-negative pathogens. This research emphasizes the potential of medicinal plants in combating ?-Lactam antibiotic resistance, offering alternative solutions to address this global health challenge.

Published

2024-04-30